论文投稿_医学论文投稿_核心期刊,职称论文投稿发表_论文部落

　　4 结论
　　明确了引起棉花黄萎病的大丽轮枝菌孢子悬浮液浓度为大于5.0×105 孢子/mL，建立了大丽轮枝菌的标准化培养和孢子悬液制备方法，甘油菌种活化培养5 d，标准培养瓶扩大培养9 d，确定了25 mL 灭菌水为适宜的孢子洗脱体积，定量制备的孢子悬浮液适合于接种棉花幼苗并引起黄萎病。在此基础上，建立了大丽轮枝菌致病相关突变体的快速筛选体系。应用该体系测试表明，1 人1 个循环7 个流程可完成1 344个突变体的快速筛选体系，鉴定周期仅54 d，工作量仅为21 人日，极大地缩短了筛选周期。同时，测试获得69 个致病力显著下降的突变体，对筛选到的致病性相关突变体进行定量菌液接种验证，结果一致。研究建立的快速筛选体系适用于T-DNA 随机插入突变体库的筛选鉴定，这将为后续大丽轮枝菌致病性相关突变体的批量筛选提供保障。
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